pET24b-Amg2
(Plasmid
#199434)
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Purposeexpresses Amg2 in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 199434 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET24b
- Backbone size w/o insert (bp) 5200
- Total vector size (bp) 5711
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAmg2
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Alt nameAM1_C0023g2
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SpeciesAcaryochloris marina MBIC11017
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Insert Size (bp)503
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MutationL159K mutation
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GenBank IDNC_009928.1 NC_009928.1
- Promoter T7
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Tag
/ Fusion Protein
- his tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET24b-Amg2 was a gift from Andrew Woolley (Addgene plasmid # 199434 ; http://n2t.net/addgene:199434 ; RRID:Addgene_199434) -
For your References section:
Engineering of bidirectional, cyanobacteriochrome-based light-inducible dimers (BICYCL)s. Jang J, Tang K, Youn J, McDonald S, Beyer HM, Zurbriggen MD, Uppalapati M, Woolley GA. Nat Methods. 2023 Feb 23. doi: 10.1038/s41592-023-01764-8. 10.1038/s41592-023-01764-8 PubMed 36823330
Map uploaded by the depositor.
