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Addgene

pcDNA3-myc3-NEDD8
(Plasmid #19943)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19943 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5437
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8
  • Alt name
    NEDD8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    245
  • GenBank ID
    NM_006156
  • Entrez Gene
    NEDD8 (a.k.a. NEDD-8)
  • Tag / Fusion Protein
    • myc3 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N51S in seq does not appear to affect function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-myc3-NEDD8 was a gift from Yue Xiong (Addgene plasmid # 19943 ; http://n2t.net/addgene:19943 ; RRID:Addgene_19943)

  • For your References section:

    The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1. Furukawa M, Zhang Y, McCarville J, Ohta T, Xiong Y. Mol Cell Biol. 2000 Nov . 20(21):8185-97. 10.1128/MCB.20.21.8185-8197.2000 PubMed 11027288
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