pcDNA3-HA-CUL1 Y42A/M43A
(Plasmid #19940)

• Depositing Lab: Yue Xiong
• Publication: Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97.

Backbone

• Vector backbone: pcDNA3-HA
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: G418

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cullin 1 Y42A/M43A
• Alt name: CUL1
• Species: H. sapiens (human)
• Insert Size (bp): 2260
• Mutation: CUL1 with a SKP1 binding mutant
• GenBank ID: NP_003583
• Entrez Gene: CUL1
• Tag / Fusion Protein:
  • HA2 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

flag tag is a variant: YPYDLPDYA

S2A mutation present but does not appear to affect function