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pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody
(Plasmid #199390)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199390 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDG-Kan
  • Backbone size w/o insert (bp) 4415
  • Total vector size (bp) 4781
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-ALFA nanobody
  • Alt name
    ALFA-ST(Super-Tight) peptide nanobody
  • Species
    Llama glama
  • Insert Size (bp)
    364
  • Promoter T5-LacO
  • Tag / Fusion Protein
    • 14xHis-Avi-SUMOEu (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer gtatcacgaggccctttcg
  • 3′ sequencing primer gttctgaggtcattactgg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody was a gift from Rebecca Voorhees (Addgene plasmid # 199390 ; http://n2t.net/addgene:199390 ; RRID:Addgene_199390)

  • For your References section:

    A nanobody-based strategy for rapid and scalable purification of human protein complexes. Stevens TA, Tomaleri GP, Hazu M, Wei S, Nguyen VN, DeKalb C, Voorhees RM, Pleiner T. Nat Protoc. 2024 Jan;19(1):127-158. doi: 10.1038/s41596-023-00904-w. Epub 2023 Nov 16. 10.1038/s41596-023-00904-w PubMed 37974029
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