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pcDNA3-HA-CUL1 K720A
(Plasmid #19939)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19939 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-HA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cullin 1 K720A
  • Alt name
    CUL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2260
  • Mutation
    CUL1 with a NEDD8 modification mutant
  • GenBank ID
    NP_003583
  • Entrez Gene
    CUL1
  • Tag / Fusion Protein
    • HA2 (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-CUL1 K720A was a gift from Yue Xiong (Addgene plasmid # 19939 ; http://n2t.net/addgene:19939 ; RRID:Addgene_19939)

  • For your References section:

    The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1. Furukawa M, Zhang Y, McCarville J, Ohta T, Xiong Y. Mol Cell Biol. 2000 Nov . 20(21):8185-97. 10.1128/MCB.20.21.8185-8197.2000 PubMed 11027288
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