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Addgene

pDest_U6_A2_CG2_typeEGF_gRNA
(Plasmid #199386)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199386 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDestTol2CG2-U6:gRNA
  • Vector type
    Tol2 destination vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nrg1 EGF sgRNA
  • Species
    D. rerio (zebrafish)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest_U6_A2_CG2_typeEGF_gRNA was a gift from William Talbot (Addgene plasmid # 199386 ; http://n2t.net/addgene:199386 ; RRID:Addgene_199386)
  • For your References section:

    Unmyelinated sensory neurons use Neuregulin signals to promote myelination of interneurons in the CNS. Lysko DE, Talbot WS. Cell Rep. 2022 Nov 15;41(7):111669. doi: 10.1016/j.celrep.2022.111669. 10.1016/j.celrep.2022.111669 PubMed 36384112