pDest_U6_A2_CG2_typeEGF_gRNA
(Plasmid
#199386)
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PurposepDEL110; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 EGF sgRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199386 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDestTol2CG2-U6:gRNA
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Vector typeTol2 destination vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenrg1 EGF sgRNA
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SpeciesD. rerio (zebrafish)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDest_U6_A2_CG2_typeEGF_gRNA was a gift from William Talbot (Addgene plasmid # 199386 ; http://n2t.net/addgene:199386 ; RRID:Addgene_199386) -
For your References section:
Unmyelinated sensory neurons use Neuregulin signals to promote myelination of interneurons in the CNS. Lysko DE, Talbot WS. Cell Rep. 2022 Nov 15;41(7):111669. doi: 10.1016/j.celrep.2022.111669. 10.1016/j.celrep.2022.111669 PubMed 36384112