pSiM1
(Plasmid #199380)

• Purpose: Encodes dual cistronic membrane localized GCaMP6s and mScarlet-I optimized for C. elegans expression
• Depositing Lab: Hang Lu
• Publication: plasmids optimized for C. elegans expression (unpublished)

Backbone

• Vector backbone: pDSP1
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GCaMP6s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
• Species: C. elegans (nematode)
• Mutation: no mutations, except added subcellular localization sequences

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pSIM1.gb
  • pSIM1.pdf

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry