pHJL3
(Plasmid
#199378)
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PurposeEncodes dual cistronic cytosolic jGCaMP7s and mScarlet-I optimized for C. elegans expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199378 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDSP1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namejGCaMP7s::SL2::mScarlet-I
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SpeciesC. elegans (nematode)
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Mutationno mutations
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHJL3 was a gift from Hang Lu (Addgene plasmid # 199378 ; http://n2t.net/addgene:199378 ; RRID:Addgene_199378)