pDSP33
(Plasmid
#199375)
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PurposeEncodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 199375 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDSP1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
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SpeciesC. elegans (nematode)
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Mutationno mutations, except added subcellular localization sequences
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDSP33 was a gift from Hang Lu (Addgene plasmid # 199375 ; http://n2t.net/addgene:199375 ; RRID:Addgene_199375)
