Skip to main content
Addgene

pTS118 His14-Avi-28xGS-anti ALFA tag nanobody
(Plasmid #199371)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199371 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDG-Kan
  • Backbone size w/o insert (bp) 4124
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-ALFA nanobody
  • Alt name
    ALFA-ST(Super-Tight) peptide nanobody
  • Species
    Llama glama
  • Insert Size (bp)
    366
  • Mutation
    WT
  • Promoter T5-LacO
  • Tag / Fusion Protein
    • 14xHis-Avi (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTS118 His14-Avi-28xGS-anti ALFA tag nanobody was a gift from Rebecca Voorhees (Addgene plasmid # 199371 ; http://n2t.net/addgene:199371 ; RRID:Addgene_199371)

  • For your References section:

    A nanobody-based strategy for rapid and scalable purification of human protein complexes. Stevens TA, Tomaleri GP, Hazu M, Wei S, Nguyen VN, DeKalb C, Voorhees RM, Pleiner T. Nat Protoc. 2024 Jan;19(1):127-158. doi: 10.1038/s41596-023-00904-w. Epub 2023 Nov 16. 10.1038/s41596-023-00904-w PubMed 37974029
Related items:
Commonly requested with: