pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS
(Plasmid
#199351)
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Purpose(Empty Backbone) Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 199351 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHAGE2
- Backbone size (bp) 7483
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Vector typeMammalian Expression, Lentiviral
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Tag
/ Fusion Protein
- TagBFP-P2A-ALFA (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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MutationWT
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Tag
/ Fusion Protein
- TagBFP-P2A-ALFA (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.03.09.531980v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS was a gift from Rebecca Voorhees (Addgene plasmid # 199351 ; http://n2t.net/addgene:199351 ; RRID:Addgene_199351) -
For your References section:
A nanobody-based strategy for rapid and scalable purification of human protein complexes. Stevens TA, Tomaleri GP, Hazu M, Wei S, Nguyen VN, DeKalb C, Voorhees RM, Pleiner T. Nat Protoc. 2024 Jan;19(1):127-158. doi: 10.1038/s41596-023-00904-w. Epub 2023 Nov 16. 10.1038/s41596-023-00904-w PubMed 37974029
