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pLenti-C-STAT1-mGFP-P2A-Puro
(Plasmid #199345)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199345 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-C-mGFP-P2A-Puro
  • Backbone size w/o insert (bp) 7750
  • Total vector size (bp) 9919
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    signal transducer and activator of transcription 1
  • Alt name
    STAT1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2247
  • GenBank ID
    NM_001205313.1
  • Entrez Gene
    Stat1 (a.k.a. 2010005J02Rik)
  • Promoter CMV
  • Tag / Fusion Protein
    • mGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-C-STAT1-mGFP-P2A-Puro was a gift from Massimo Mallardo (Addgene plasmid # 199345 ; http://n2t.net/addgene:199345 ; RRID:Addgene_199345)

  • For your References section:

    The Lack of STING Impairs the MHC-I Dependent Antigen Presentation and JAK/STAT Signaling in Murine Macrophages. Caiazza C, Brusco T, D'Alessio F, D'Agostino M, Avagliano A, Arcucci A, Ambrosino C, Fiume G, Mallardo M. Int J Mol Sci. 2022 Nov 17;23(22):14232. doi: 10.3390/ijms232214232. 10.3390/ijms232214232 PubMed 36430709
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