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pTol2_claudink:Cas9green; control_gRNA
(Plasmid #199336)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199336 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDestTol2CG2-U6:gRNA
  • Vector type
    Tol2 destination vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    claudink
  • Species
    D. rerio (zebrafish)

Gene/Insert 2

  • Gene/Insert name
    Cas9

Gene/Insert 3

  • Gene/Insert name
    control sgRNA
  • Insert Size (bp)
    20

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol2_claudink:Cas9green; control_gRNA was a gift from William Talbot (Addgene plasmid # 199336 ; http://n2t.net/addgene:199336 ; RRID:Addgene_199336)

  • For your References section:

    Unmyelinated sensory neurons use Neuregulin signals to promote myelination of interneurons in the CNS. Lysko DE, Talbot WS. Cell Rep. 2022 Nov 15;41(7):111669. doi: 10.1016/j.celrep.2022.111669. 10.1016/j.celrep.2022.111669 PubMed 36384112
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