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Addgene

pUREF-EX
(Plasmid #199331)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199331 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF-BOS-EX
  • Backbone size (bp) 5277
  • Modifications to backbone
    An SV40 promoter-driven puromycin resistance gene was inserted.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUREF-EX was a gift from Rikiro Fukunaga (Addgene plasmid # 199331 ; http://n2t.net/addgene:199331 ; RRID:Addgene_199331)

  • For your References section:

    A simple, rapid, and efficient method for generating multigene-knockout culture cells by the CRISPR/Cas9 system. Fujii T, Inoue N, Nobeyama T, Inoue J, Ogasawara S, Otani Y, Fujii S, Ito C, Fukunaga R. Genes Cells. 2023 Mar 2. doi: 10.1111/gtc.13021. 10.1111/gtc.13021 PubMed 36861495
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