pNMSB109
(Plasmid
#199322)
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PurposeCombine with Gal4 to direct CaMBI 300 expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199322 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHW394
- Total vector size (bp) 6335
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert name15xUAS::delta pes-10::::CaMBI::let-858 3'UTR
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SpeciesC. elegans (nematode)
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Insert Size (bp)3789
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MutationOrange CaMBI with 300 nM KD for calcium
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GenBank IDOQ576356
- Promoter 15xUAS:::delta pes-10
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13rev
- 3′ sequencing primer M13fwd (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNMSB109 was a gift from Michael Krieg (Addgene plasmid # 199322 ; http://n2t.net/addgene:199322 ; RRID:Addgene_199322) -
For your References section:
Neural engineering with photons as synaptic transmitters. Porta-de-la-Riva M, Gonzalez AC, Sanfeliu-Cerdan N, Karimi S, Malaiwong N, Pidde A, Morales-Curiel LF, Fernandez P, Gonzalez-Bolivar S, Hurth C, Krieg M. Nat Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9. 10.1038/s41592-023-01836-9 PubMed 37024651