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Addgene

pcDNA3-RIPK3[S227A]-FLAG
(Plasmid #199318)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199318 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Addgene
  • Total vector size (bp) 7014
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Receptor-interacting serine/threonine-protein kinase 3
  • Alt name
    RIPK3
  • Species
    H. sapiens (human)
  • Mutation
    changed Serine 227 to Alanine
  • Entrez Gene
    RIPK3 (a.k.a. RIP3)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-RIPK3[S227A]-FLAG was a gift from Adrian Press (Addgene plasmid # 199318 ; http://n2t.net/addgene:199318 ; RRID:Addgene_199318)

  • For your References section:

    RIPK3 promoter hypermethylation in hepatocytes protects from bile acid-induced inflammation and necroptosis. Hoff J, Xiong L, Kammann T, Neugebauer S, Micheel JM, Gassler N, Bauer M, Press AT. Cell Death Dis. 2023 Apr 18;14(4):275. doi: 10.1038/s41419-023-05794-0. 10.1038/s41419-023-05794-0 PubMed 37072399
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