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pNMSB84
(Plasmid #199309)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199309 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFJ150
  • Backbone size w/o insert (bp) 9131
  • Total vector size (bp) 13756
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    6039
  • Mutation
    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elegans codon optimized jRGECO1a with 1 intron
  • GenBank ID
    OQ576354
  • Promoter flp-18

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNMSB84 was a gift from Michael Krieg (Addgene plasmid # 199309 ; http://n2t.net/addgene:199309 ; RRID:Addgene_199309)

  • For your References section:

    Neural engineering with photons as synaptic transmitters. Porta-de-la-Riva M, Gonzalez AC, Sanfeliu-Cerdan N, Karimi S, Malaiwong N, Pidde A, Morales-Curiel LF, Fernandez P, Gonzalez-Bolivar S, Hurth C, Krieg M. Nat Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9. 10.1038/s41592-023-01836-9 PubMed 37024651
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