pBMN HA-NDP52
(Plasmid #199307)

• Purpose: For expression of the mammalian NDP52 tagged with HA
• Depositing Lab: Michael Lazarou
• Publication: Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021.

Backbone

• Vector backbone: pBMN
• Backbone size w/o insert (bp): 5028
• Total vector size (bp): 6368
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NDP52
• Alt name: CALCOCO2
• Species: H. sapiens (human)
• Insert Size (bp): 1551
• GenBank ID: NM_005831.5
• Entrez Gene: CALCOCO2 (a.k.a. NDP52)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pBMN 5'
• 3′ sequencing primer: pBMN 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry