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pFastBac Dual wt p110α/p85α
(Plasmid #199302)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199302 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac Dual
  • Backbone manufacturer
    ThermoFisher Scientific
  • Backbone size w/o insert (bp) 5238
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His-tagged p110α wt and Avi-tagged p85α wt
  • Species
    H. sapiens (human)
  • Entrez Gene
    PIK3CA (a.k.a. CCM4, CLAPO, CLOVE, CWS5, HMH, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha)
  • Entrez Gene
    PIK3R1 (a.k.a. AGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha)
  • Tags / Fusion Proteins
    • Avi (N terminal on insert)
    • His

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene was cloned by Peter Shepherd's Lab University of Auckland, NZ

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

received and added Avi-tag

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac Dual wt p110α/p85α was a gift from Peter Vogt (Addgene plasmid # 199302 ; http://n2t.net/addgene:199302 ; RRID:Addgene_199302)

  • For your References section:

    Publisher Correction: Stereo- and regiodefined DNA-encoded chemical libraries enable efficient tumour-targeting applications. Favalli N, Bassi G, Pellegrino C, Millul J, De Luca R, Cazzamalli S, Yang S, Trenner A, Mozaffari NL, Myburgh R, Moroglu M, Conway SJ, Sartori AA, Manz MG, Lerner RA, Vogt PK, Scheuermann J, Neri D. Nat Chem. 2021 Jul;13(7):714. doi: 10.1038/s41557-021-00742-x. 10.1038/s41557-021-00742-x PubMed 34103693
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