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Addgene

pGEX-ARF
(Plasmid #19929)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19929 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-KG
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 5006
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cyclin-dependent kinase inhibitor 2A p19ARF
  • Alt name
    ARF
  • Alt name
    p19
  • Alt name
    p19ARF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    560
  • GenBank ID
    pfam07392
  • Entrez Gene
    CDKN2A (a.k.a. ARF, CAI2, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pGEX
  • 3′ sequencing primer pGEX
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that there are several isoforms of ARF. Addgene encourages scientists to read the associated paper carefully before requesting this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-ARF was a gift from Yue Xiong (Addgene plasmid # 19929 ; http://n2t.net/addgene:19929 ; RRID:Addgene_19929)

  • For your References section:

    ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways. Zhang Y, Xiong Y, Yarbrough WG. Cell. 1998 Mar 20. 92(6):725-34. 10.1016/S0092-8674(00)81401-4 PubMed 9529249
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