pCMV-Cyclin D1
(Plasmid #19927)

• Depositing Lab: Yue Xiong
• Publication: Watanabe et al Proc Natl Acad Sci U S A. 1998 Feb 17. 95(4):1392-7.

Backbone

• Vector backbone: pCMV
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: G418

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cyclin D1
• Alt name: CCND1
• Species: H. sapiens (human)
• Insert Size (bp): 1325
• GenBank ID: NM_053056
• Entrez Gene: CCND1 (a.k.a. BCL1, D11S287E, PRAD1, U21B31)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry