Skip to main content
Addgene

pEF_PE2
(Plasmid #199267)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199267 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PE2-P2A-mp53DD
  • Alt name
    SV40_bpNLS-SpCas9(H840A)-SGGSx2-XTEN16-SGGSx2-MMLV_RT(D200N, T306K, W313F, T330P, L603W)-SV40_bpNLS
  • Alt name
    Trp53 carboxy-terminal dominant-negative fragment
  • Species
    M. musculus (mouse), Synthetic; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus
  • Insert Size (bp)
    6716
  • Mutation
    Δ40-903
  • GenBank ID
    NM_011640

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    PE2 is from Addgene Plasmid #132775

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF_PE2 was a gift from Ting Zhou (Addgene plasmid # 199267 ; http://n2t.net/addgene:199267 ; RRID:Addgene_199267)

  • For your References section:

    Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells. Li M, Zhong A, Wu Y, Sidharta M, Beaury M, Zhao X, Studer L, Zhou T. Nat Commun. 2022 Oct 27;13(1):6354. doi: 10.1038/s41467-022-34045-7. 10.1038/s41467-022-34045-7 PubMed 36302757
Related items:
Commonly requested with: