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Addgene

AW01_pU.CAG.eSpCas9(1.1).rBGpA
(Plasmid #199255)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199255 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 4965
  • Total vector size (bp) 9360
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    High specificity eSpCas9(1.1) nuclease
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4266
  • Mutation
    K848A K1003A R1060A
  • Promoter CAG promoter
  • Tags / Fusion Proteins
    • 3XFLAG epitope (N terminal on insert)
    • SV40 NLS (N terminal on insert)
    • Nucleoplasmin NLS (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The SpCas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system, mutated to K848A, K1003A and R1060A to improve targeting specificity (Slaymaker et al., Science 2016 351:84-88) was inserted into a pCAG.rBGpA expression plasmid backbone.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AW01_pU.CAG.eSpCas9(1.1).rBGpA was a gift from Manuel Goncalves (Addgene plasmid # 199255 ; http://n2t.net/addgene:199255 ; RRID:Addgene_199255)
  • For your References section:

    Probing the impact of chromatin conformation on genome editing tools. Chen X, Rinsma M, Janssen JM, Liu J, Maggio I, Goncalves MA. Nucleic Acids Res. 2016 Jun 8. pii: gkw524. 10.1093/nar/gkw524 PubMed 27280977