SPDK3959
(Plasmid #199246)

• Purpose: Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA vector with AtPDS3 target for editing Arabidopsis PDS3 gene
• Depositing Lab: Savithramma Dinesh-Kumar
• Publication: Nagalakshmi et al Plant Physiol. 2022 Jun 27;189(3):1241-1245. doi: 10.1093/plphys/kiac159.

Backbone

• Vector backbone: pCAMBIS0390 T-DNA vector
• Backbone size w/o insert (bp): 6812
• Total vector size (bp): 11056
• Vector type: T-DNA vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: We prefer to grow in DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Modified TRV RNA2 with PEBV promoter, AtPDS3 target and AtIleu-tRNA mobile RNA sequence
• Insert Size (bp): 2956
• Promoter: PEBV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: SacI (unknown if destroyed)
• 5′ sequencing primer: CATAATTATACTGATTTGTCTCTCG
• 3′ sequencing primer: caaaagacttaccgatcaatcaag

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry