pSH624-ssr
(Plasmid #199241)

• Purpose: Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E
• Depositing Lab: Susanne Häußler
• Publication: Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41596-023-00882-z.

Backbone

• Vector backbone: pSEVA624
• Backbone manufacturer: SEVA collection
• Backbone size w/o insert (bp): 5089
• Total vector size (bp): 5888
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: single-stranded DNA recombineering
• Alt name: ssr
• Alt name: T1E_1405
• Species: Pseudomonas putida DOT-T1E
• Insert Size (bp): 799
• GenBank ID: WP_014859796.1 NC_018220.1
• Promoter: lacIq-Ptrc

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AvrII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: PTRC-99A-FOR
• 3′ sequencing primer: F24

Resource Information

• Supplemental Documents:
  • pSH624-ssr_final.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry