pS848∙CsR
(Plasmid
#199240)
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PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199240 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepS448∙CsR
- Backbone size w/o insert (bp) 8398
- Total vector size (bp) 9897
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Vector typeCRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegRNA scaffold - Gm resistance cassette - incP oriT
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SpeciesSynthetic
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Insert Size (bp)1503
- Promoter PEM7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SanDI (not destroyed)
- 3′ cloning site FseI (not destroyed)
- 5′ sequencing primer SEVA_T0-F
- 3′ sequencing primer PS4 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pS848∙CsR was a gift from Susanne Häußler (Addgene plasmid # 199240 ; http://n2t.net/addgene:199240 ; RRID:Addgene_199240) -
For your References section:
An expanded CRISPR-Cas9-assisted recombineering toolkit for engineering genetically intractable Pseudomonas aeruginosa isolates. Pankratz D, Gomez NO, Nielsen A, Mustafayeva A, Gur M, Arce-Rodriguez F, Nikel PI, Haussler S, Arce-Rodriguez A. Nat Protoc. 2023 Oct 5. doi: 10.1038/s41596-023-00882-z. 10.1038/s41596-023-00882-z PubMed 37798358