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pET32a-hNRG1a
(Plasmid #199235)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199235 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET32a(+)
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5851
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Neuregulin
  • Alt name
    ARIA
  • Alt name
    NRG1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    207
  • Entrez Gene
    NRG1 (a.k.a. ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, MST131, MSTP131, NDF, NRG1-IT2, SMDF)
  • Promoter T7
  • Tag / Fusion Protein
    • TrxA-6xHis-S-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET32a-hNRG1a was a gift from Nilson Zanchin (Addgene plasmid # 199235 ; http://n2t.net/addgene:199235 ; RRID:Addgene_199235)

  • For your References section:

    Functional EGF domain of the human neuregulin 1alpha produced in Escherichia coli with accurate disulfide bonds. Ferreira AS, Lopacinski A, Batista M, Hiraiwa PM, Bueno NF, Guimaraes BG, Zanchin NIT. Mol Biol Rep. 2022 Dec;49(12):11715-11727. doi: 10.1007/s11033-022-07956-3. Epub 2022 Oct 5. 10.1007/s11033-022-07956-3 PubMed 36198848
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