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Addgene

pET32a-hEGF
(Plasmid #199231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199231 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET32a(+)
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5863
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Epidermal growth factor, partial
  • Alt name
    HOMG4
  • Alt name
    URG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    159
  • Entrez Gene
    EGF (a.k.a. HOMG4, URG)
  • Promoter T7
  • Tag / Fusion Protein
    • TrxA-6xHis-S-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET32a-hEGF was a gift from Nilson Zanchin (Addgene plasmid # 199231 ; http://n2t.net/addgene:199231 ; RRID:Addgene_199231)

  • For your References section:

    A toolkit for recombinant production of seven human EGF family growth factors in active conformation. Ferreira AS, Lopacinski A, Batista M, Hiraiwa PM, Guimaraes BG, Zanchin NIT. Sci Rep. 2022 Mar 23;12(1):5034. doi: 10.1038/s41598-022-09060-9. 10.1038/s41598-022-09060-9 PubMed 35322149
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