pcDNA3-myc3-CUL4B
(Plasmid #19922)

• Depositing Lab: Yue Xiong
• Publication: Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Backbone

• Vector backbone: pcDNA3-myc3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5437
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cullin 4B
• Alt name: CUL4B
• Species: H. sapiens (human)
• Insert Size (bp): 4394
• GenBank ID: NM_003588
• Entrez Gene: CUL4B (a.k.a. CUL-4B, MRXHF2, MRXS15, MRXSC, SFM2)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xmyc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: -- (not destroyed)
• 3′ cloning site: -- (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry