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Addgene

1/2-NORAD-(A)4x
(Plasmid #199208)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199208 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ACTB-(A)4x
  • Backbone size w/o insert (bp) 4394
  • Total vector size (bp) 5520
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    1/2-NORAD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2644
  • Mutation
    4 copies of Riboglow tag after stop codon
  • GenBank ID
    NORAD
  • Promoter CMV
  • Tag / Fusion Protein
    • Riboglow RNA tag (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer 5'-CGCAAATGGGCGGTAGGCGTG-3'
  • 3′ sequencing primer 5'-GAAATTTGTGATGCTATTGC-3'
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Amy Palmer (Addgene plasmid # 112058) and Roy Parker (pRP877)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ACTB-(A)4x was a gift from Amy Palmer (Addgene plasmid # 112058). 1/2-NORAD-(A)4x was constructed using standard cloning procedures from pRP877. pRP877 was a gift from Roy Parker. NORAD-Riboglow truncations were built by replacing the ACTB sequence in plasmid ACTB-(A)4x with the corresponding NORAD truncation sequence from pRP877 by Life Scientists' Service Center, LifeSct LLC.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1/2-NORAD-(A)4x was a gift from Esther Braselmann (Addgene plasmid # 199208 ; http://n2t.net/addgene:199208 ; RRID:Addgene_199208)

  • For your References section:

    Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM). Sarfraz N, Moscoso E, Oertel T, Lee HJ, Ranjit S, Braselmann E. Nat Commun. 2023 Feb 16;14(1):867. doi: 10.1038/s41467-023-36531-y. 10.1038/s41467-023-36531-y PubMed 36797241
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