1/2-NORAD-(A)4x
(Plasmid #199208)

• Purpose: 1/2 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)
• Depositing Lab: Esther Braselmann
• Publication: Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi: 10.1038/s41467-023-36531-y.

Backbone

• Vector backbone: ACTB-(A)4x
• Backbone size w/o insert (bp): 4394
• Total vector size (bp): 5520
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 1/2-NORAD
• Species: H. sapiens (human)
• Insert Size (bp): 2644
• Mutation: 4 copies of Riboglow tag after stop codon
• GenBank ID: NORAD
• Promoter: CMV
• Tag / Fusion Protein:
  • Riboglow RNA tag (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: 5'-CGCAAATGGGCGGTAGGCGTG-3'
• 3′ sequencing primer: 5'-GAAATTTGTGATGCTATTGC-3'

Resource Information

• Supplemental Documents:
  • 1:2-NORAD-(A)4x.ape
• A portion of this plasmid was derived from a plasmid made by: Amy Palmer (Addgene plasmid # 112058) and Roy Parker (pRP877)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ACTB-(A)4x was a gift from Amy Palmer (Addgene plasmid # 112058). 1/2-NORAD-(A)4x was constructed using standard cloning procedures from pRP877. pRP877 was a gift from Roy Parker. NORAD-Riboglow truncations were built by replacing the ACTB sequence in plasmid ACTB-(A)4x with the corresponding NORAD truncation sequence from pRP877 by Life Scientists' Service Center, LifeSct LLC.