pBMN_HA-TBK1 (kinase dead - D135N)
(Plasmid #199206)

• Purpose: Used for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997)
• Depositing Lab: Sascha Martens
• Publication: Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021.

Backbone

• Vector backbone: pBMN
• Backbone size w/o insert (bp): 5029
• Total vector size (bp): 7216
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TBK1
• Alt name: NF-kappa-B-activating kinase
• Alt name: T2K
• Alt name: NAK
• Species: H. sapiens (human)
• Insert Size (bp): 2187
• Mutation: D135N
• GenBank ID: AF191838
• Entrez Gene: TBK1 (a.k.a. FTDALS4, IIAE8, NAK, T2K)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: AATGGTATAGTGCACCGTAATATCAAGCCAGGAAAT
• 3′ sequencing primer: TCCTAAAGACAGTCAACGTTGCGAAGGC

Resource Information

• Supplemental Documents:
  • SMC1997. pBMN HA-TBK1_D135N (KD).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.08.14.503930v1 for bioRxiv preprint.