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Addgene

pHD-T2A-split-intein-Gal4[21-881]-C-int
(Plasmid #199204)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199204 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Derived from pCRISPaint-T2A-Gal4-3xP3-RFP
  • Total vector size (bp) 7195
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    T2A-split-intein-Gal4[21-881]-C-int
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    4287

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHD-T2A-split-intein-Gal4[21-881]-C-int was a gift from Norbert Perrimon (Addgene plasmid # 199204 ; http://n2t.net/addgene:199204 ; RRID:Addgene_199204)
  • For your References section:

    split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80. Ewen-Campen B, Luan H, Xu J, Singh R, Joshi N, Thakkar T, Berger B, White BH, Perrimon N. Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2304730120. doi: 10.1073/pnas.2304730120. Epub 2023 Jun 5. 10.1073/pnas.2304730120 PubMed 37276389