pHD-T2A-split-intein-Gal4[21-881]-C-int
(Plasmid
#199204)
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PurposeTo create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 199204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneDerived from pCRISPaint-T2A-Gal4-3xP3-RFP
- Total vector size (bp) 7195
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameT2A-split-intein-Gal4[21-881]-C-int
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SpeciesD. melanogaster (fly)
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Insert Size (bp)4287
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHD-T2A-split-intein-Gal4[21-881]-C-int was a gift from Norbert Perrimon (Addgene plasmid # 199204 ; http://n2t.net/addgene:199204 ; RRID:Addgene_199204) -
For your References section:
split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80. Ewen-Campen B, Luan H, Xu J, Singh R, Joshi N, Thakkar T, Berger B, White BH, Perrimon N. Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2304730120. doi: 10.1073/pnas.2304730120. Epub 2023 Jun 5. 10.1073/pnas.2304730120 PubMed 37276389
