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Addgene

pFastBac K-Flag M33
(Plasmid #1992)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1992 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4775
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    M33
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1560
  • Mutation
    Kozak sequence added before ATG
  • Entrez Gene
    Cbx2 (a.k.a. M33, MOD2, pc)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer Polyhedrin forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Kozak modified mouse M33 cDNA, N-terminal flag. (kingston #1390)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac K-Flag M33 was a gift from Robert Kingston (Addgene plasmid # 1992 ; http://n2t.net/addgene:1992 ; RRID:Addgene_1992)

  • For your References section:

    Propagation of silencing; recruitment and repression of naive chromatin in trans by polycomb repressed chromatin. Lavigne M, Francis NJ, King IF, Kingston RE. Mol Cell 2004 Feb 13;13(3):415-25. 10.1016/S1097-2765(04)00006-1 PubMed 14967148
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