pDropIn-T2A-ZpGal4DBD
(Plasmid
#199199)
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PurposeInsert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digest
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199199 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePCR Blunt II - TOPO
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Backbone manufacturerInvitrogen
- Total vector size (bp) 5768
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameT2A-ZpGal4DBD
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SpeciesD. melanogaster (fly)
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Insert Size (bp)2250
Resource Information
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A portion of this plasmid was derived from a plasmid made byInsert derived from pBPZpGAL4DBDUw - Addgene 26233
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDropIn-T2A-ZpGal4DBD was a gift from Norbert Perrimon (Addgene plasmid # 199199 ; http://n2t.net/addgene:199199 ; RRID:Addgene_199199) -
For your References section:
split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80. Ewen-Campen B, Luan H, Xu J, Singh R, Joshi N, Thakkar T, Berger B, White BH, Perrimon N. Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2304730120. doi: 10.1073/pnas.2304730120. Epub 2023 Jun 5. 10.1073/pnas.2304730120 PubMed 37276389