pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R
(Plasmid
#199198)
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PurposeInsert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digest
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199198 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePCR Blunt II - TOPO
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Backbone manufacturerInvitrogen
- Total vector size (bp) 9231
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameT2A-split-intein-Gal4[21-881]-C-int-G418R
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Insert Size (bp)5713
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R was a gift from Norbert Perrimon (Addgene plasmid # 199198 ; http://n2t.net/addgene:199198 ; RRID:Addgene_199198) -
For your References section:
split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80. Ewen-Campen B, Luan H, Xu J, Singh R, Joshi N, Thakkar T, Berger B, White BH, Perrimon N. Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2304730120. doi: 10.1073/pnas.2304730120. Epub 2023 Jun 5. 10.1073/pnas.2304730120 PubMed 37276389