pGBT9-γ2 ear
(Plasmid
#199178)
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PurposeExpression of GAL4 DNA-binding domain (BD)-AP-1 γ2 ear domain fusion protein in yeast (yeast two-hybrid assays)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199178 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGBT9
- Backbone size w/o insert (bp) 5524
- Total vector size (bp) 5868
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAP-1 γ2 ear domain
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SpeciesH. sapiens (human)
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Insert Size (bp)351
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Entrez GeneAP1G2 (a.k.a. G2AD)
- Promoter ADH1
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Tag
/ Fusion Protein
- GAL4-DNA binding domain fragment (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer GAL4 DNA binding domain forward
- 3′ sequencing primer GAL4 DNA binding domain reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
AP-1 γ2 ear domain: residues 670-785 of human AP-1 γ2
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGBT9-γ2 ear was a gift from Juan Bonifacino (Addgene plasmid # 199178 ; http://n2t.net/addgene:199178 ; RRID:Addgene_199178) -
For your References section:
Divalent interaction of the GGAs with the Rabaptin-5-Rabex-5 complex. Mattera R, Arighi CN, Lodge R, Zerial M, Bonifacino JS. EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.1093/emboj/cdg015. 10.1093/emboj/cdg015 PubMed 12505986