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Addgene

pGBT9-GGA3-GAE
(Plasmid #199176)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199176 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGBT9
  • Backbone size w/o insert (bp) 5524
  • Total vector size (bp) 6213
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GGA3-GAE domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    693
  • Entrez Gene
    GGA3
  • Promoter ADH1
  • Tag / Fusion Protein
    • GAL4-DNA binding domain fragment (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamH1 (destroyed during cloning)
  • 5′ sequencing primer GAL4 DNA binding domain forward
  • 3′ sequencing primer GAL4 DNA binding domain reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GAE domain: similar to the γ-adaptin ear domain
This GGA3-GAE domain construct comprises residues 494-723 of human GGA3 (includes part of the hinge domain)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBT9-GGA3-GAE was a gift from Juan Bonifacino (Addgene plasmid # 199176 ; http://n2t.net/addgene:199176 ; RRID:Addgene_199176)

  • For your References section:

    Divalent interaction of the GGAs with the Rabaptin-5-Rabex-5 complex. Mattera R, Arighi CN, Lodge R, Zerial M, Bonifacino JS. EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.1093/emboj/cdg015. 10.1093/emboj/cdg015 PubMed 12505986
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