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Addgene

pGBT9-GGA2-GAE
(Plasmid #199175)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199175 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGBT9
  • Backbone size w/o insert (bp) 5524
  • Total vector size (bp) 5912
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GGA2-GAE domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    386
  • Mutation
    Contains Lys 545 Gln substitution
  • Entrez Gene
    GGA2 (a.k.a. VEAR)
  • Promoter ADH1
  • Tag / Fusion Protein
    • GAL4-DNA binding domain fragment (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GAL4 DNA binding domain forward
  • 3′ sequencing primer GAL4 DNA binding domain reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GAE domain: similar to the γ-adaptin ear domain
GGA2-GAE domain: residues 489-613 of human GGA2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBT9-GGA2-GAE was a gift from Juan Bonifacino (Addgene plasmid # 199175 ; http://n2t.net/addgene:199175 ; RRID:Addgene_199175)

  • For your References section:

    Divalent interaction of the GGAs with the Rabaptin-5-Rabex-5 complex. Mattera R, Arighi CN, Lodge R, Zerial M, Bonifacino JS. EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.1093/emboj/cdg015. 10.1093/emboj/cdg015 PubMed 12505986
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