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pET-6xHis-GFP(+36)-DL4
(Plasmid #199168)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199168 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETDuet
  • Total vector size (bp) 5896

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Growth and protein expression after induction is improved at 25 °C.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP(+36)-DL4
  • Alt name
    inclusion body forming variant of GFP(+36)
  • Insert Size (bp)
    744
  • Mutation
    E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q90R, E100K, D112K, T115K, D127R, E134K, I138R, D143K, E152R, N159K, Q167R, N174K, E182K, D200R, deletion 201-206, D207R, Q214R, A216K, N222K, T240K, M243R, L246R
  • Promoter T7
  • Tag / Fusion Protein
    • MGHHHHHHGGA (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    This gene was synthesized and cloned into the plasmid by GenScript.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-6xHis-GFP(+36)-DL4 was a gift from Allie Obermeyer (Addgene plasmid # 199168 ; http://n2t.net/addgene:199168 ; RRID:Addgene_199168)

  • For your References section:

    Formation of Biomolecular Condensates in Bacteria by Tuning Protein Electrostatics. Yeong V, Werth EG, Brown LM, Obermeyer AC. ACS Cent Sci. 2020 Dec 23;6(12):2301-2310. doi: 10.1021/acscentsci.0c01146. Epub 2020 Nov 12. 10.1021/acscentsci.0c01146 PubMed 33376791
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