pET-6xHis-GFP(0)
(Plasmid
#199166)
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Purposeused to express a neutral GFP variant in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 199166 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepETDuet
- Total vector size (bp) 5896
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP(0)
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Insert Size (bp)744
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MutationE16R, I138R, D207R, N222K, L246R
- Promoter T7
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Tag
/ Fusion Protein
- MGHHHHHHGGA (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThis gene was synthesized and cloned into the plasmid by GenScript.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-6xHis-GFP(0) was a gift from Allie Obermeyer (Addgene plasmid # 199166 ; http://n2t.net/addgene:199166 ; RRID:Addgene_199166) -
For your References section:
Formation of Biomolecular Condensates in Bacteria by Tuning Protein Electrostatics. Yeong V, Werth EG, Brown LM, Obermeyer AC. ACS Cent Sci. 2020 Dec 23;6(12):2301-2310. doi: 10.1021/acscentsci.0c01146. Epub 2020 Nov 12. 10.1021/acscentsci.0c01146 PubMed 33376791
Map uploaded by the depositor.
