pET-6xHis-GFP(+6)
(Plasmid #199160)

• Purpose: used to express a supercharged GFP variant in E. coli
• Depositing Lab: Allie Obermeyer
• Publication: Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-2310. doi: 10.1021/acscentsci.0c01146. Epub 2020 Nov 12.

Backbone

• Vector backbone: pETDuet
• Total vector size (bp): 5896

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Growth and protein expression after induction is improved at 25 °C.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP(+6)
• Insert Size (bp): 744
• Mutation: E16R, D86K, D112K, D127R, I138R, D207R, N222K, L246R
• Promoter: T7
• Tag / Fusion Protein:
  • MGHHHHHHGGA (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Supplemental Documents:
  • copy-of-pet-6xhis-pos6gfp.gb
• A portion of this plasmid was derived from a plasmid made by: This gene was synthesized and cloned into the plasmid by GenScript.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry