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Addgene

RB814
(Plasmid #199142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS314
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 6599
  • Vector type
    Yeast Expression
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    100 bp barcoding cassette
  • Species
    Synthetic
  • Insert Size (bp)
    100

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site ClaI (unknown if destroyed)
  • 5′ sequencing primer GTGCCGTAAAGCACTAAATCGG
  • 3′ sequencing primer GTTACCTCACTCATTAGGCACCCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

After restriction digestion with NgoMIV, this plasmid can be used to transform Candida strains which would barcode it with unique DNA barcodes

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RB814 was a gift from Richard Bennett (Addgene plasmid # 199142 ; http://n2t.net/addgene:199142 ; RRID:Addgene_199142)
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