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Addgene

pDT4
(Plasmid #199120)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199120 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Total vector size (bp) 8864
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    synthetic transcription template
  • Mutation
    mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to introduce a second BsaI site

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDT4 was a gift from Jeff Gelles (Addgene plasmid # 199120 ; http://n2t.net/addgene:199120 ; RRID:Addgene_199120)

  • For your References section:

    Recycling of bacterial RNA polymerase by the Swi2/Snf2 ATPase RapA. Inlow K, Tenenbaum D, Friedman LJ, Kondev J, Gelles J. Proc Natl Acad Sci U S A. 2023 Jul 11;120(28):e2303849120. doi: 10.1073/pnas.2303849120. Epub 2023 Jul 5. 10.1073/pnas.2303849120 PubMed 37406096
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