pCDNA3-myc3-FBW5 delta F
(Plasmid
#19912)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 19912 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3-myc3
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5437
-
Vector typeMammalian Expression
-
Selectable markersG418
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameF-box and WD repeat domain containing 5 delta F
-
Alt nameFBW5 delta F
-
Alt nameFBXW5 delta F
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1752
-
Mutationdeleted aa1-42 (F-box)
-
GenBank IDNM_018998
-
Entrez GeneFBXW5 (a.k.a. Fbw5)
-
Tag
/ Fusion Protein
- myc3 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI/ XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
F-Box Deletion Mutant
(L45M mutation present but does not affect function)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCDNA3-myc3-FBW5 delta F was a gift from Yue Xiong (Addgene plasmid # 19912 ; http://n2t.net/addgene:19912 ; RRID:Addgene_19912) -
For your References section:
WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase. Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y. Genes Dev. 2008 Apr 1. 22(7):866-71. 10.1101/gad.1624008 PubMed 18381890