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Addgene

pDT2
(Plasmid #199119)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199119 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFW11 Tet
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
  • Species
    Synthetic
  • Mutation
    mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to introduce a second BsaI site
  • GenBank ID
    KT326913

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Debora Tenanbaum

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDT2 was a gift from Jeff Gelles (Addgene plasmid # 199119 ; http://n2t.net/addgene:199119 ; RRID:Addgene_199119)

  • For your References section:

    Recycling of bacterial RNA polymerase by the Swi2/Snf2 ATPase RapA. Inlow K, Tenenbaum D, Friedman LJ, Kondev J, Gelles J. Proc Natl Acad Sci U S A. 2023 Jul 11;120(28):e2303849120. doi: 10.1073/pnas.2303849120. Epub 2023 Jul 5. 10.1073/pnas.2303849120 PubMed 37406096
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