pKI1
(Plasmid #199118)

• Purpose: Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling
• Depositing Lab: Jeff Gelles
• Publication: Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;120(28):e2303849120. doi: 10.1073/pnas.2303849120. Epub 2023 Jul 5.

Backbone

• Vector backbone: pQE80L
• Backbone size w/o insert (bp): 4500
• Total vector size (bp): 8168
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H6-SNAP-RapA
• Species: E. coli
• Insert Size (bp): 3474
• GenBank ID:
• Tags / Fusion Proteins:
  • SNAP-tag (N terminal on insert)
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • Chung_k2l_1_KI_plasmid_pLann.gbk
• A portion of this plasmid was derived from a plasmid made by: H6-RapA plasmid from Katsuhiko Murakami

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry