pcDNA3-HA2-DDB1
(Plasmid #19909)

• Depositing Lab: Yue Xiong
• Publication: Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Backbone

• Vector backbone: pcDNA3-HA2
• Backbone size w/o insert (bp): 5512
• Vector type: Mammalian Expression
• Selectable markers: G418

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Damage-specific DNA binding protein 1
• Alt name: DDB1
• Species: H. sapiens (human)
• Insert Size (bp): 3420
• Mutation: please see depositor comment below
• GenBank ID: NM_001923
• Entrez Gene: DDB1 (a.k.a. DDBA, UV-DDB1, WHIKERS, XAP1, XPCE, XPE, XPE-BF)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: -- (not destroyed)
• 3′ cloning site: -- (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's quality control result found mutations I253T, I258L and M679V in DDB1 when compared to the Genbank NM_001923. The plasmid should function as described in the original publication (Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71).