pGLDV_12 [18e4G]
(Plasmid #199075)

• Purpose: (Empty Backbone) Even assembly vector with sfGFP dropout for Even level assemblies
• Depositing Labs: Charlie Gilbert, Nili Ostrov, Cultivarium Tools
• Publication: Gilbert et al bioRxiv 2023.05.19.541510

Backbone

• Vector backbone: n/a
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): E. coli Zymo 10B
• Growth instructions: Supplement LB growth medium with 2% glucose (w/v)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Jim Haseloff (Pollak et al. 2018 https://doi.org/10.1111/nph.15625)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For standard propagation, supplement LB growth medium with 2% glucose (w/v) to repress sfYFP dropout expression, otherwise escape mutants may arise.