pCDNA3-myc3-FBW5
(Plasmid #19905)

• Depositing Lab: Yue Xiong
• Publication: Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Backbone

• Vector backbone: pcDNA3-myc3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5437
• Vector type: Mammalian Expression
• Selectable markers: G418

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: F-box and WD repeat domain containing 5
• Alt name: FBW5
• Alt name: FBXW5
• Species: H. sapiens (human)
• Insert Size (bp): 1887
• GenBank ID: NM_018998
• Entrez Gene: FBXW5 (a.k.a. Fbw5)
• Tag / Fusion Protein:
  • myc3 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI/ XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry