pGL1_3
(Plasmid
#199035)
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PurposeLevel 1 plasmid, barcoded ORI
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199035 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGLDV_5
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. coli Zymo 10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepAM?1 ORI plus NGS barcode 3
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SpeciesSynthetic
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL1_3 was a gift from Charlie Gilbert & Nili Ostrov & Cultivarium Tools (Addgene plasmid # 199035 ; http://n2t.net/addgene:199035 ; RRID:Addgene_199035) -
For your References section:
A scalable framework for high-throughput identification of functional origins of replication in non-model bacteria. Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z, Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N. bioRxiv 2023.05.19.541510 10.1101/2023.05.19.541510